Gas chromatographie determination of extracellular metabolites produced by baker’s yeast during glucose-induced acidification |
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Authors: | M Wurst K Sigler A Knotková |
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Institution: | (1) Department of Experimental Mycology, Czechoslovak Academy of Sciences, 142 20 Prague 4;(2) Department of Cell Physiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4 |
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Abstract: | Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker’s yeast supplied with
glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography
on Chromosorb G-AW-DMCS with 2.5 % SE-52 in a programmed temperature interval of 80–220 °C (3 °C/min) made it possible to
separate organic acids (lactic, pyruvic, succinic, glyceric, fumaric, malic), polyols (glycerol, arabinitol) and sugars (glucose).
The yeast was found to extrude glycerol, lactic, malic, and succinic acid. |
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