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| 四川地区阴道毛滴虫内人型支原体的PCR检测 | |
| 引用本文： | 朱晓燕,王雅静,毕世樑,张仁刚. 四川地区阴道毛滴虫内人型支原体的PCR检测[J]. 四川动物, 2009, 28(6) |
| 作者姓名： | 朱晓燕 王雅静 毕世樑 张仁刚 |
| 作者单位： | 1. 川北医学院寄生虫学教研室,四川南充,637007 2. 四川大学华西基础医学与法医学院寄生虫学教研室 3. 四川大学华西第二医院妇产科 |
| 摘 要： | 从临床上分离获得20株阴道毛滴虫虫株,经纯化培养后,提取基因组DNA.以人型支原体16S rDNA序列设计特异性引物,利用PCR技术检测阴道毛滴虫内的人型支原体,结果有13株为人型支原体阳性,感染率为65%,表明阴道毛滴虫与人型支原体的共生关系在中国四川具有普遍性. |
| 关 键 词： | 阴道毛滴虫 人型支原体 |
PCR Research of Mycoplasma hominis in Trichomonas vaginalis Cells of Sichuan Province |
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| ZHU Xiao-yan,WANG Ya-jing,BI Shi-liang,ZHANG Ren-gang. PCR Research of Mycoplasma hominis in Trichomonas vaginalis Cells of Sichuan Province[J]. Sichuan Journal of Zoology, 2009, 28(6) | |
| Authors: | ZHU Xiao-yan WANG Ya-jing BI Shi-liang ZHANG Ren-gang |
| Abstract: | 20 isolates of Trichomonas vaginalis were collected from clinical patients in hospital. After pure cultivation, genomic DNA of T. vaginalis was extracted. A pair of specific 16S rDNA primers was designed based on the sequence of Mycoplasma hominis. M. hominis in T. vaginalis cells were detected by PCR. Altogether 20 isolates of T. vaginalis were collected, 13 out of 20 T. vaginalis isolates harboured M. hominis, and the symbiosis ratio between T. vaginalis and M. hominis was up to 65%. It suggested that the symbiotic relationship between T. vaginalis and M. hominis occurred commonly in Sichuan, China. |
| Keywords: | PCR |
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