Aminoglycoside suppression at UAG, UAA and UGA codons in Escherichia coli and human tissue culture cells |
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Authors: | Robin Martin Anne E Mogg Louise A Heywood Lars Nitschke and Julian F Burke |
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Institution: | (1) Biochemistry Laboratory, Biological Sciences, University of Sussex, BN1 9QG Falmer, UK;(2) Present address: Institute for Biochemistry, University of Freiburg, D-7800 Freiburg, Federal Republic of Germany |
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Abstract: | Summary We have compared the suppression of nonsense mutations by aminoglycoside antibiotics inEscherichia coli and in human 293 cells. Six nonsense alleles of the chloramphenicol acetyl transferase (cat) gene, in the vector pRSVcat, were suppressed by growth in G418 and paromomycin. Readthrough at UAG, UAA and UGA codons was monitored with enzyme assays
for chloramphenicol acetyl transferase (CAT), in stably transformed bacteria and during transient expression from the same
plasmid in human 293 tissue culture cells. We have found significant differences in the degree of suppression amongst three
UAG codons and two UAA codons in different mRNA contexts. However, the pattern of these effects are not the same in the two
organisms. Our data suggest that context effects of nonsense suppression may operate under different rules inE. coli and human cells. |
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Keywords: | Nonsense suppression Context effects Aminoglycosides |
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