Production of fibronectin by HUH6 C15 cell line established from a human hepatoblastoma |
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| Authors: | M Tanaka K Kawamura M Fang K Higashino S Kishimoto H Nakabayashi J Sato |
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| Affiliation: | 1. The Third Department of Internal Medicine, Osaka University School of Medicine, Osaka 553, Japan;2. Division of Pathology, Cancer Institute, Okayama University School of Medicine, Okayama 700, Japan |
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| Abstract: | Fibronectin was detected by indirect immunofluorescence on the cell surfaces of HUH6 C15 cells, established from a human hepatoblastoma and maintained with serum-free RPMI 1640 medium. Fibronectin synthesized by HUH6 Cl5 was purified by gelatin-Sepharose affinity chromatography and compared with human plasma fibronectin in respect to molecular weight, electrophoretic mobility and antigenicity. Fibronectin synthesized by this cell line was proved to be identical with human plasma fibronectin. |
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