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Inoculation of Rhizobium (VR-1 and VA-1) induces an increasing growth and metal accumulation potential in Vigna radiata and Vigna angularis L. growing under fly-ash
Authors:S.K. ChaudharyM. Inouhe  U.N. RaiK. Mishra  D.K. Gupta
Affiliation:a Ecotoxicology and Bioremediation Group, National Botanical Research Institute, Lucknow 226001, India
b Department of Biology and Environmental Sciences, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan
c Department of Botany, University of Lucknow, Lucknow 226007, India
d Departamento de Bioquimica, Biologia Cellular y Molicular de Plantas, Estacion Experimental Del Zaidin, CSIC, Granada 18008, Spain
Abstract:Fly-ash-tolerant Rhizobium strains were isolated from plants grown in fly-ash-contaminated soil, axenically under laboratory conditions. Saplings of both plants were raised in N2-free Jenson medium and inoculated with 2.6 × 108 cell ml−1 and 5.2 × 108 cell ml−1 of culture after 10 d of growth. Plants were transferred into 100% fly-ash under natural condition. Rhizobium-inoculated plants grown on 100% fly-ash showed marked increase in relation to root-shoot length, biomass yield, photosynthetic pigment, protein content and nodulation frequency compared to uninoculated plant grown in control (100% fly-ash). Inoculation of fly-ash-tolerant Rhizobium increased the accumulation of Fe, Zn, Cu Cd and Cr in different tissues vis-à-vis enhanced translocation of metals to the aboveground part of plant. Although inoculation of fly-ash-tolerant Rhizobium strains (VR-1 and VA-1) enhanced the translocation of more Fe to shoot parts, nevertheless, the amount of Rhizobium inoculants supplied to the plant was found to be very important since it has a positive role in increasing plant growth through increased N2 supply via nitrogenase activity. Results suggest that an integrated approach employing biotechnological means and inoculation of plants with host-specific fly-ash-tolerant Rhizobium strain may prove a stimulus to a fly-ash management programme.
Keywords:Fly-ash   Rhizobium   Metals   Photosynthetic pigments   Protein
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