A PCR-based sex-determination assay in cattle based on the bovine amelogenin locus |
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| Authors: | S Ennis T F Gallagher |
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| Affiliation: | Department of Animal Husbandry and Production, Faculty of Veterinary Medicine and Department of Botany, Faculty of Science, University College Dublin, Dublin, Ireland |
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| Abstract: | A method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 μ1 of blood or biopsies taken from 6–7-day-old embryos. The primers amplify a 280 bp band in females and a 280 and 217bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR failure. |
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| Keywords: | amelogenin PCR sex determination bovine embryo biopsies. |
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