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Aqueous two-phase systems for molecular weight analyses
Authors:Tawanda Mushuku  M A N Benhura  Rajni Kaul  Mats Larsson  Bo Mattiasson
Institution:(1) Department of Biotechnology, Chemical Center, Lund University, S-22100 Lund, Sweden;(2) Department of Biochemistry, University of Zimbabwe, Harare, Zimbabwe;(3) Present address: Swedish Meat Research Institute, P.O. Box 504, S-224 24 Kävlinge, Sweden
Abstract:Summary The potential of aqueous two-phase systems for the estimation of molecular weights of proteins and enzyme-inhibitor complexes has been demonstrated. The method is simple and rapid, and employs extremely small amounts of the test sample. It is based on the measurement of the phase transition behaviour on dilution of the system as a function of the molecular weight of the molecule or complex under study.
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