<Emphasis Type="Italic">Pseudoruegeria marinistellae</Emphasis> sp. nov., isolated from an unidentified starfish in Sanya,China

A taxonomic study was carried out on a Gram-stain negative, rod-shaped, non-flagellated, and facultatively anaerobic bacterial strain designated as strain SF-16^T, which was isolated from an unidentified starfish in Sanya, China. Strain SF-16^T was found to be 5.0–7.0 μm long, and oxidase and catalase positive. Cell growth was observed at pH 6.0–8.5 (optimum, 7.0–8.0), temperatures of 10–41 °C (optimum, 25–30 °C), and salinities of 0–12 % (optimum, 3.0–6.0 %). The predominant fatty acids (>20 %) were found to be C_18:1 ω7c and/or C_18:1 ω6c (summed feature 8). Ubiquinone 10 was identified as the predominant quinone for strain SF-16^T. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unidentified lipids, and three unidentified phospholipids. The DNA G+C content of the genomic DNA of strain SF-16^T was determined to be 63.0 mol%. Phylogenetic analysis based on the 16S rRNA gene showed that strain SF-16^T belongs to the genus Pseudoruegeria and is closely related to Pseudoruegeria sabulilitoris GJMS-35^T (98.42 % similarity). The ANI value between strain SF-16^T and P. sabulilitoris GJMS-35^T was found to be 74.98 %, and DNA–DNA hybridization value was 21.1 ± 2.3 % in silico and 57 % in vitro. Based on the low level of the genetic relatedness, phylogenetic and phenotypic data, a novel species Pseudoruegeria marinistellae sp. nov. is proposed. The type strain is SF-16^T (=MCCC 1K01155^T = KCTC 42910^T).