<Emphasis Type="Italic">Streptomyces luozhongensis</Emphasis> sp. nov., a novel actinomycete with antifungal activity and antibacterial activity

A novel actinomycete strain, designated TRM 49605^T, was isolated from a desert soil sample from Lop Nur, Xinjiang, north-west China, and characterised using a polyphasic taxonomic approach. The strain exhibited antifungal activity against the following strains: Saccharomyces cerevisiae, Curvularia lunata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium citrinum, Candida albicans and Candida tropicalis; Antibacterial activity against Bacillus subtilis, Staphylococcus epidermidis and Micrococcus luteus; and no antibacterial activity against Escherichia coli. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 49605^T to the genus Streptomyces. Strain TRM 49605^T shows high sequence similarities to Streptomyces roseolilacinus NBRC 12815^T (98.62 %), Streptomyces flavovariabilis NRRL B-16367^T (98.45 %) and Streptomyces variegatus NRRL B-16380^T (98.45 %). Whole cell hydrolysates of strain TRM 49605^T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and galactose, glucose, xylose and mannose as the major whole cell sugars. The major fatty acids in strain TRM 49605^T were identified as iso C_16:0, anteiso C_15:0, C_16:0 and Summed Feature 5 as defined by MIDI. The main menaquinones were identified as MK-9(H₄), MK-9(H₆), MK-9(H₈) and MK-10(H₆). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. The G+C content of the genomic DNA was determined to be 71.2 %. The DNA–DNA relatedness between strain TRM 49605^T and the phylogenetically related strain S. roseolilacinus NBRC 12815^T was 60.12 ± 0.06 %, which is lower than the 70 % threshold value for delineation of genomic prokaryotic species. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain TRM 49605^T (=CCTCC AA2015026^T = KCTC 39666^T) should be designated as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces luozhongensis sp. nov. is proposed.