Online monitoring of immunoaffinity-based depletion of high-abundance blood proteins by UV spectrophotometry using enhanced green fluorescence protein and FITC-labeled human serum albumin |
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| Authors: | Kyunggon Kim Jiyoung Yu Hophil Min Hyunsoo Kim Byungwook Kim Hyeong Gon Yu Youngsoo Kim |
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| Institution: | 1.Department of Biomedical Sciences,Seoul National University, College of Medicine,Seoul,Korea;2.Department of Biomedical Engineering,Seoul National University, College of Medicine,Seoul,Korea;3.Department of Ophthalmology,Seoul National University, College of Medicine,Seoul,Korea;4.Cancer Research Institute, Seoul National University College of Medicine,Seoul,Korea |
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| Abstract: | Background The removal of high-abundance proteins from plasma is an efficient approach to investigating flow-through proteins for biomarker
discovery studies. Most depletion methods are based on multiple immunoaffinity methods available commercially including LC
columns and spin columns. Despite its usefulness, high-abundance depletion has an intrinsic problem, the sponge effect, which
should be assessed during depletion experiments. Concurrently, the yield of depletion of high-abundance proteins must be monitored
during the use of the depletion column. To date, there is no reasonable technique for measuring the recovery of flow-through
proteins after depletion and assessing the capacity for capture of high-abundance proteins. |
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