Crystallization and preliminary X-ray studies of glutathione synthetase from Escherichia coli B |
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| Authors: | H Kato H Yamaguchi Y Hata T Nishioka Y Katsube J Oda |
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| Institution: | Institute for Chemical Research, Kyoto University, Japan. |
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| Abstract: | The glutathione synthetase from Escherichia coli B has been crystallized from 27% saturated ammonium sulfate solution (pH 5.5). The crystals are hexagonal, space group P6(2)22 or P6(4)22. The cell dimensions are a = b = 88.0 A, c = 164.2 A, and gamma = 120 degrees. The enzyme is a tetramer (Mr = 143,000) with 222 symmetry, and the asymmetric unit contains one subunit molecule (Mr = 35,600). The crystals diffract to at least 2.5 A resolution. |
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