Efficient CNS gene delivery by intravenous injection |
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Authors: | Louboutin Jean-Pierre Chekmasova Alena A Marusich Elena Chowdhury J Roy Strayer David S |
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Institution: | Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. jplouboutin@hotmail.com |
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Abstract: | We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS. |
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