Improvement of a Venus-based bimolecular fluorescence complementation assay to visualize bFos-bJun interaction in living cells |
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| Authors: | Nakagawa Chika Inahata Kazuto Nishimura Shigenori Sugimoto Kenji |
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| Institution: | Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. cnakagawa@bioinfo.osakafu-u.ac.jp |
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| Abstract: | Bimolecular fluorescence complementation (BiFC) assay makes it possible to visualize protein-protein interactions in living cells. In this assay, Venus, a bright-yellow variant of green fluorescent protein, is known to produce fluorescent backgrounds due to non-specific interactions. In this study we found that the V150A mutation increased by 8.6-fold the signal-to-noise ratio in the BiFC assay of bFos-bJun interaction. |
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