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A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based upon salicylate signalling pathways and the modulation of carbon,antioxidant and photo‐oxidative metabolism
Authors:JAVIER SÁNCHEZ‐MARTÍN  JIM HEALD  ALISON KINGSTON‐SMITH  ANA WINTERS  DIEGO RUBIALES  MARILUZ SANZ  LUIS A. J. MUR  ELENA PRATS
Affiliation:1. Institute for Sustainable Agriculture, CSIC, Córdoba, Spain;2. Institute of Biological, Environmental and Rural Sciences, University of Aberystwyth, Aberystwyth, UK;3. Institute of General Organic Chemistry, CSIC, Madrid, Spain
Abstract:Although a wealth of information is available on the induction of one or several drought‐related responses in different species, little is known of how their timing, modulation and crucially integration influence drought tolerance. Based upon metabolomic changes in oat (Avena sativa L.), we have defined key processes involved in drought tolerance. During a time course of increasing water deficit, metabolites from leaf samples were profiled using direct infusion–electrospray mass spectroscopy (DI‐ESI‐MS) and high‐performance liquid chromatography (HPLC) ESI‐MS/MS and analysed using principal component analysis (PCA) and discriminant function analysis (DFA). The involvement of metabolite pathways was confirmed through targeted assays of key metabolites and physiological experiments. We demonstrate an early accumulation of salicylic acid (SA) influencing stomatal opening, photorespiration and antioxidant defences before any change in the relative water content. These changes are likely to maintain plant water status, with any photoinhibitory effect being counteracted by an efficient antioxidant capacity, thereby representing an integrated mechanism of drought tolerance in oats. We also discuss these changes in relation to those engaged at later points, consequence of the different water status in susceptible and resistant genotypes.
Keywords:metabolomics  photorespiration  salicylic acid
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