Potential for biparental cytoplasmic inheritance in Jasminum officinale and Jasminum nudiflorum |
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Authors: | Sodmergen H H Bai J X He H Kuroiwa S Kawano T Kuroiwa |
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Institution: | (1) College of Life Sciences, Peking University, Beijing 100871, China, CN;(2) Department of Biology, Jiujiang Teachers College,;(3) Department of Biological Sciences, Graduate School of Science, University of Tokyo, |
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Abstract: | Mature Jasminum officinale and J. nudiflorum pollen grains were stained with 4′,6-diamidino-2-phenylindole (DAPI) and examined by epifluorescence microscopy. The pollen
grains were found to be trinucleate, and the sperm cells in both species contained a large number of epifluorescent spots
that corresponded to cytoplasmic DNA aggregates (nucleoids). The nucleoids of J. nudiflorum were observed to be dimorphic under the epifluorescence microscope, indicating that the sperm cells might contain both plastid
and mitochondrial DNA. The nucleoids of J. officinale presented a similar appearance when stained with DAPI, but electron microscopic examination of the sperm cells revealed that
they contained both plastids and mitochondria. When analyzed by DNA immunogold electron microscopy, gold particles were detected
on both plastids and mitochondria. These findings demonstrated the preservation of plastid and mitochondrial DNA in mature
sperm cells and thus the potential for biparental cytoplasmic inheritance in J. officinale and J. nudiflorum.
Received: 8 August 1997 / Revision accepted: 25 February 1998 |
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Keywords: | Plastid Mitochondrion Biparental cytoplasmic inheritance Jasminum officinale Jasminum nudiflorum |
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