In vitro translation of drosophila heat-shock and non-heat-shock mRNAs in heterologous and homologous cell-free systems |
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Authors: | Christiane Krüger Bernd-Joachim Benecke |
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Institution: | Lehrstuhl für Biochemie Ruhr-Universität D-463 Bochum, Federal Republic of Germany |
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Abstract: | Upon heat shock, Drosophila Kc cells still contain normal cellular messenger RNAs in the cytoplasm. The distribution of these 25°C mRNAs between polysomes and the postpolysomal fraction of heat-shocked cells appears unaltered as compared with control cells. The translatability of these normal cellular messages isolated from heat-shocked and non-heat-shocked Kc cells is unaltered when analyzed by in vitro translation in the rabbit reticulocyte lysate. In contrast, homologous cell-free translation systems obtained from Kc cells effectively discriminate between the in vitro translation of normal cellular messages and heat-shock-specific mRNAs. In particular, a cell-free system from heat-shocked Drosophila Kc cells almost completely shuts down the translation of 25°C messenger RNA species, whereas the translatability of heat-shock-specific messenger RNA appears to be unaffected. |
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