| 人乳头瘤病毒16型早期基因E6、E7的克隆及表达 |
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| 引用本文： | 余黎,韩平,安静,姜英,周旭.人乳头瘤病毒16型早期基因E6、E7的克隆及表达[J].微生物学免疫学进展,2007,35(2):7-11. |
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| 作者姓名： | 余黎 韩平 安静 姜英 周旭 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 高危人乳头瘤病毒16型的感染与宫颈癌的发病密切相关。HPV16E6和E7蛋白在大多数HPV16相关宫颈癌及其癌前病变中持续表达,因此E6和E7蛋白可作为制备HPV16相关肿瘤及其癌前病变治疗性疫苗的靶抗原〔2〕。采用套式PCR方法,从宫颈癌患者组织中扩增出E6、E7基因并成功构建了包含E6、E7的表达质粒PET-E6、PET-E7。SDS聚丙烯酰胺凝胶电泳和Western-blotting分析结果表明,外源基因E6,E7在T7启动子控制下可获得稳定表达。
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| 关 键 词： | 人乳头瘤病毒 克隆基因 表达 |
| 文章编号： | 1005-5673（2007）02-0007-05 |
| 收稿时间： | 2006-08-09 |
| 修稿时间： | 2006-08-092006-12-29 |
Cloning and expression of recombinant PET-HPV16E6, PET-HPV16E7 in E.coli |
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| YU Li, HAN Ping ,AN Jing ,et al..Cloning and expression of recombinant PET-HPV16E6, PET-HPV16E7 in E.coli[J].Progress In Microbiology and Immunology,2007,35(2):7-11. |
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| Authors: | YU Li HAN Ping AN Jing |
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| Institution: | Lanzhou Institute of Biological Products, Lanzhou 730046, China |
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| Abstract: | Human papillomavirus(HPV) type 16(HPV16) infection in human is associated with most cervical cancers,and expression of the early oncogenic proteins E6 and E7 is required to maintain the transformed state of the tumor cell.Therefore,E6 and E7 are appropriate tumor-specific antigen and target for vaccine-based treatment of HPV-16-associated malignancies.Using Nested Polymerase Chain Reaction(PCR),amplify HPVE6 and E7 gene,construct expressing plasmids PET-E6 and PET-E7 which containing the early gene E6 and E7.SDS-PAGE and Western-blot result showed that the cloned gene were expressed under the control of T7 promoter in E.coli DE3. |
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| Keywords: | HPV Gene cloning Expression |
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