The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidised bed reactors |
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| Authors: | M Reiter O Hohenwarter T Gaida N Zach C Schmatz G Blüml F Weigang K Nilsson H Katinger |
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| Institution: | (1) Department of Biotechnology, Institute of Applied Microbiology (IAM), University of Agriculture, 1190 Vienna, Austria;(2) Percell Biolytica AB, Lund, Sweden |
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| Abstract: | A fluidised bed system for the cultivation of mammalian cells on a new type of macroporous gelatin microcarrier is described. The volumetric cell densities achieved under controlled conditions for two  standard cell lines (VERO, CHO) were one order of magnitude higher compared to conventional techniques using spherical microcarriers. The system can be potentially used for both anchorage dependent and independent cells. |
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| Keywords: | macroporous microcarriers fluidised bed immobilisation anchorage dependent cells |
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