珍稀药用和观赏植物地涌金莲的组织培养和快速繁殖

以地涌金莲(Musella lasiocarpa)吸芽为外植体建立了有效的快繁体系。外植体经灭菌处理后在MS 6-BA4.0 mg L-1 NAA 0.2 mg L-1 维生素C 150 mg L-1 10%椰子乳 3%蔗糖培养基上能进行不定芽的诱导和增殖,培养60 d后每个芽平均能产生4.10个不定芽。第6代增殖后,丛生芽的增殖系数可达4.23。生根培养基以1/2MS NAA1.0 mg L-1 AC 50 mg L-1的效果较好。以沙:泥炭土:珍珠岩=1:1:1为基质移栽试管苗,成活率达到93.5%以上。经过12个月的组织培养已生产10 000多株试管苗。

Tissue Culture and Rapid Propagation of Musella lasiocarpa, a Rare Chinese Medicinal and Ornamental Herb

An efficient and rapid micropropagation system by using auxiliary buds as explants has been established for Musella lasiocarpa.Murashige and Skoog(MS) medium supplemented with 4.0 mg L-1 6-benzylaminopurine,0.2 mg L-1 1-napthaleneacetic acid,150 mg L-1 vitamin C,10% coconut milk and 3% sucrose was suitable for shoot induction and proliferation from divided buds.Each explant produced an average of 4.10 shoots after 60-day culture.After the sixth subculture,the shoot proliferation rate of plantlets was 4.23-fold.The half-strength MS medium supplemented with 1.0 mg L-1 indole-3-butyric acid and 150 mg L-1 activated charcoal was suitable for rooting.When micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand,sieved peat and perlite(1:1:1;v/v) in a greenhouse,93.5% of the plantlets survived.About 10 000 plantlets were produced successfully for field transfer after 12 months of culture initiation.This production system is useful for ex situ conservation and large-scale multiplication of M.lasiocarpa.