| 涅斯捷连科氏菌特异性引物PCR分子鉴定 |
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| 引用本文： | 职晓阳,杨玲玲,王芸,唐蜀昆,娄恺,李文均.涅斯捷连科氏菌特异性引物PCR分子鉴定[J].微生物学报,2008,48(5):644-650. |
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| 作者姓名： | 职晓阳 杨玲玲 王芸 唐蜀昆 娄恺 李文均 |
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| 作者单位： | 1. 云南大学微生物研究所,教育部微生物多样性可持续利用重点实验室,昆明 650091
2. 新疆农业科学院微生物研究所,乌鲁木齐,830091 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家自然科学基金
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教育部科学技术研究重点项目
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教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 涅斯捷连科氏菌属的建立基于对Micrococcus halobius的再分类,这是一类广泛分布于高盐土壤环境的革兰氏阳性细菌,属放线菌类.在对我国西部盐湖环境放线菌的生物多样性及分类学研究中,大量类似菌株被分离.目的]为了对其进行快速鉴定,特别是筛选出属于优势类群的涅斯捷连科氏菌,方法]本研究根据前人以报道的方法设计了一对针对其16S rRNA基因的特异性PCR引物(Nes1/Nes2),结果]并通过部分典型菌和野生菌株的PCR实验验证,结合16S rRNA基因的测序验证,证明了Nes1/Nes2的PCR反应有效性及其对涅斯捷连科氏菌的特异性.结论]利用该引物可以快速准确的对涅斯捷连科氏菌进行鉴定.
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| 关 键 词： | 涅斯捷连科氏菌 属特异性引物 分子鉴定 Nesterenkonia Genus-specific primers Molecular identification 氏菌 特异性引物 分子鉴定 genus novel Partial sequencing specificity reference strains Results synthesized polymerase chain reaction based gene sequences rapid identification species Objective |
| 文章编号： | 0001-6209(2008)05-0644-07 |
| 收稿时间： | 2007/11/12 0:00:00 |
| 修稿时间： | 2007年11月12 |
Genus-specific PCR for molecular identification of novel
isolates of the genus Nesterenkonia |
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| Xiaoyang Zhi,Lingling Yang,Yun Wang,Shukun Tang,Kai Lou and Wenjun Li.Genus-specific PCR for molecular identification of novel
isolates of the genus Nesterenkonia[J].Acta Microbiologica Sinica,2008,48(5):644-650. |
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| Authors: | Xiaoyang Zhi Lingling Yang Yun Wang Shukun Tang Kai Lou and Wenjun Li |
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| Institution: | Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute
of Microbiology, Yunnan University, Kunming 650091, China;Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute
of Microbiology, Yunnan University, Kunming 650091, China;Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi 830091, China;Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute
of Microbiology, Yunnan University, Kunming 650091, China;Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi 830091, China;Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute
of Microbiology, Yunnan University, Kunming 650091, China |
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| Abstract: | Objective and Method]: For rapid identification of Nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences. Results and Conclusion]: The genus specificity of these primers was validated with reference strains as well as with wild-type isolates. Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia. |
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| Keywords: | Nesterenkonia Genus-specific primers Molecular identification |
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