| 胶片吸附的激光显微切割技术及其在蛋白质组研究中的应用 |
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| 引用本文： | 吴惠玲,邹强.胶片吸附的激光显微切割技术及其在蛋白质组研究中的应用[J].生物化学与生物物理进展,2001,28(6):919-921. |
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| 作者姓名： | 吴惠玲 邹强 |
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| 作者单位： | 第三军医大学免疫学教研室,全军免疫学研究所,重庆,400038 |
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| 摘 要： | 组织显微切割在特定细胞亚群的比较研究中起着重要作用.新近发展的胶片吸附激光显微切割技术能从复杂的生物组织中快速准确地分离纯化出特定细胞亚群.在这项技术中，透明的乙烯乙酸乙烯基酯热塑性胶片覆盖于病理组织切片表面，CO2激光束特异性作用于目的细胞群表面的胶片，胶片对细胞较强的吸附作用使得选择性自动移取目的细胞群成为可能.目前，这项技术已在蛋白质组分析中获得成功应用，并有望对其产生较深远的影响.
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| 关 键 词： | 显微切割 胶片吸附的激光显微切割技术 蛋白质组分析 |
| 收稿时间： | 2001/1/18 0:00:00 |
| 修稿时间： | 2001年1月18日 |
Laser Capture Microdissection (LCM) with its Applications in Proteome Analysis |
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| WU Hui-Ling and ZOU Qiang.Laser Capture Microdissection (LCM) with its Applications in Proteome Analysis[J].Progress In Biochemistry and Biophysics,2001,28(6):919-921. |
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| Authors: | WU Hui-Ling and ZOU Qiang |
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| Institution: | Department of Immunology in the Third Military Medical University, Institute of Immunology, Chongqing 400038, China;Department of Immunology in the Third Military Medical University, Institute of Immunology, Chongqing 400038, China |
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| Abstract: | Tissue microdissection is potentially one of the most useful techniques in the comparative investigations of specific cells. A novel laser capture microdissection (LCM) has been developed to procure precisely the single cells from complex normal and diseased tissues, in a rapid and practical manner. In this technique, a transparent thermoplastil film(ethylene vinyl acetate polymer)is applied to the surface of the tissue section on a standard glass histopathology slide; a carbon dioxide laser pulse then specifically activates the film above the cells of interest. Strong focal adhesion allows selective procurement of the targeted cells. Now LCM system had been available in proteome analysis and is likely to have a profound impact on proteome analysis. |
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| Keywords: | microdissection laser capture microdissection proteome analysis |
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