Interaction and fusion dynamics between cellular blebs |
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| Institution: | 1. Department of Mechanical Engineering, The University of Hong Kong, Hong Kong;2. HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Shenzhen, Guangdong, China;3. Department of Engineering Mechanics, Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province, Zhejiang University, Hangzhou, Zhejiang, China;1. Institute of Metal Physics of UB RAS, S. Kovalevskaya str., 18, 620990 Ekaterinburg, Russia;2. Institute of Natural Sciences and Mathematics, Ural Federal University, Mira str. 19, Ekaterinburg, Russia;1. Department of Mathematics, University of California Davis, Davis, California;2. Department of Mechanical and Aerospace Engineering, University of California San Diego, San Diego, California;3. Department of Biochemistry and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California;4. Courant Institute and Department of Biology, New York University, New York, New York;1. Center for Biomedical Engineering, University of Science and Technology of China, Hefei 230027, China;2. Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China;3. School of Biology, Food and Environment Engineering, Hefei University, Hefei 230601, China;4. Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230027, China;5. Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China;1. Department of Mathematics, Applied Mathematics, and Statistics, Case Western Reserve University, Cleveland, Ohio;2. Department of Mathematics, University of California Davis, Davis, California;2. Department of Physics and Astronomy, University College London, London, United Kingdom;3. London Centre for Nanotechnology, University College London, London, United Kingdom;4. Department of Cell and Developmental Biology, University College London, London, United Kingdom |
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| Abstract: | Membrane blebbing, as a mechanism for cells to regulate their internal pressure and membrane tension, is believed to play important roles in processes such as cell migration, spreading and apoptosis. However, the fundamental question of how different blebs interact with each other during their life cycles remains largely unclear. Here, we report a combined theoretical and experimental investigation to examine how the growth and retraction of a cellular bleb are influenced by neighboring blebs as well as the fusion dynamics between them. Specifically, a boundary integral model was developed to describe the shape evolution of cell membrane during the blebbing/retracting process. We showed that a drop in the intracellular pressure will be induced by the formation of a bleb whose retraction then restores the pressure level. Consequently, the volume that a second bleb can reach was predicted to heavily depend on its initial weakened size and the time lag with respect to the first bleb, all in quantitative agreement with our experimental observations. In addition, it was found that as the strength of membrane-cortex adhesion increases, the possible coalescence of two neighboring blebs changes from smooth fusion to abrupt coalescence and eventually to no fusion at all. Phase diagrams summarizing the dependence of such transition on key physical factors, such as the intracellular pressure and bleb separation, were also obtained. |
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| Keywords: | Cell blebbing Membrane deformation Cell adhesion |
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