Flow Cytometry Demonstrates Bacteriocin-Induced Injury to Listeria monocytogenes |
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| Authors: | Avril J Swarts John W Hastings Robert F Roberts Alexander von Holy |
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| Institution: | (1) Department of Microbiology, University of the Witwatersrand, Private Bag 3, Wits 2050, South Africa , ZA;(2) Department of Genetics, University of Natal, Bag X01, Scottsville 3209, South Africa , ZA;(3) Department of Food Science, Pennsylvania State University, University Park, PA 16802, USA , US |
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| Abstract: | Flow cytometry was used to study the effect of the bacteriocin leucocin B-TA11a on Listeria (L.) monocytogenes. Mixed proportions of dead and live control populations were analyzed by flow cytometry to determine detection limits of
the Dead/Live Baclight Bacterial Viability KitTM. High correlations for flow cytometric detection of defined proportions of live or dead cells in mixtures between 10 and
100% of dead (r2 = 0.97) or live (r2 = 0.99) cells were obtained. However, mixtures containing less than 10% of either live or dead control cells gave correlations
below 0.72. The growth of L. monocytogenes in the absence and presence of leucocin B-TA11a was analyzed by flow cytometry with Baclight, plate counts, and optical density measurements. Although leucocin B-TA11a initially inhibited listerial growth, the
uptake of both Baclight dyes suggested that cells remained viable but became leaky, possibly indicating bacteriocin-induced pore formation in
the target membranes.
Received: 30 June 1997 / Accepted: 20 October 1997 |
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