Characteristics of nitric oxide-evoked [H]taurine release from cerebral cortical neurons |
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Authors: | Da-Zhi Chen Seitaro Ohkuma Kinya Kuriyama |
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Institution: | Department of Pharmacology, Kyoto Prefectural University of Medicine, Kamikyo-Ku, Kyoto 602, Japan |
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Abstract: | Pharmacological characteristics of 3H]taurine release evoked by nitric oxide (NO) were investigated using mouse cerebral cortical neurons in primary culture. NO generators such as S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SNP) dose-dependently increased 3H]taurine release from neurons. Such stimulatory effects of NO generators were completely abolished by hemoglobin, a NO radical scavenger, indicating that these 3H]taurine releases might be due to NO liberated from SNAP and SNP. Sodium withdrawal from incubation buffer significantly inhibited the SNAP- and SNP-induced 3H]taurine releases, whereas the removal of calcium showed no alterations in the 3H]taurine release evoked by NO generators. β-Alanine and guanidinoethane sulfonate, inhibitors of carrier-mediated taurine transport system, inhibited the SNAP- and SNP-evoked releases of 3H]taurine in a dose-dependent manner. These results indicate that the NO-evoked 3H]taurine release from cerebral cortical neurons is mediated by the reverse process of sodium-dependent carrier-mediated taurine transport system. |
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