Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system |
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Authors: | Karla Martínez Ramón de Anda Georgina Hernández Adelfo Escalante Guillermo Gosset Octavio T Ramírez and Francisco G Bolívar |
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Institution: | (1) Departamento de Ingenier?a Celular y Biocat?lisis, Instituto de Biotecnolog?a, Universidad Nacional Aut?noma de M?xico (UNAM), Av. Universidad 2001, CP 62210 Cuernavaca, Morelos, M?xico;(2) Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog?a, Universidad Nacional Aut?noma de M?xico (UNAM), Av. Universidad 2001, CP 62210 Cuernavaca, Morelos, M?xico |
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Abstract: | Background
Escherichia coli strains lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) are capable of coutilizing glucose
and other carbon sources due to the absence of catabolite repression by glucose. In these strains, the lack of this important
regulatory and transport system allows the coexistence of glycolytic and gluconeogenic pathways. Strains lacking PTS have
been constructed with the goal of canalizing part of the phosphoenolpyruvate (PEP) not consumed in glucose transport to the
aromatic pathway. The deletion of the ptsHIcrr operon inactivates PTS causing poor growth on this sugar; nonetheless, fast growing mutants on glucose have been isolated
(PB12 strain). However, there are no reported studies concerning the growth potential of a PTS- strain in mixtures of different carbon sources to enhance the production of aromatics compounds. |
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Keywords: | |
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