Structural and functional characterization of a pollen-specific promoter <Emphasis Type="Italic">NTPp13</Emphasis> in tobacco |
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| Authors: | A Yang S Zhang Sh Liu Q Zhao G Pan |
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| Institution: | 1.Institute of Tobacco Sciences,Chinese Academy of Agricultural Sciences,Qingdao, Shandong,China;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing,China;3.Capital Normal University,Beijing,China;4.Key Laboratory of Crop Genetic Resources and Improvement,Sichuan Agricultural University,Ya’an,China |
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| Abstract: | A novel 407 bp nucleotide sequence NTPp13 was isolated from tobacco (Nicotiana tabacum L.) by PCR, its structure and function were characterized. The NTPp13 sequence was highly homologous with the pollen-specific expression promoter Zm13 from maize (Zea mays L.) and contained some key motifs which controlled pollen-specific expression. The NTPp13 was fused to the β-glucuronidase (GUS) reporter gene and transferred into tobacco. Analysis of the transgenic plants revealed
that this putative promoter fragment was sufficient to direct GUS expression specifically in the anther, exactly in the pollen
and pollen tube, and that GUS activity reached the maximum at the stage of pollen grain began to separate. Further study showed
that the expression of NTPp13 sequence at pollen was stable at the range of temperature measured. These data suggested that the NTPp13 sequence was likely the essential element of promoter region of an unknown pollen-specific gene from tobacco. |
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