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Restriction map and location of mutations on the genome of bacteriophage Hp1c1 of Haemophilus influenzae Rd |
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| Authors: | W P Fitzmaurice J J Scocca |
| Affiliation: | Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205 U.S.A. Tel. (301) 955-3667 |
| Abstract: | A physical map of the 32.4-kb chromosome of the Haemophilus influenzae bacteriophage Hp1c1 has been constructed, using the cleavage sites of eight restriction endonucleases. Two temperature-sensitive mutations have also been localized on the phage chromosome. The phage DNA exhibited an affinity for the specific DNA receptor of Haemophilus transformation approx. 1.5-fold higher than that obtained with bulk chromosomal DNA of H. influenzae. |
| Keywords: | Transformation transfection DNA recognition specific DNA receptors lysogenic phage bp base pairs cfu colony-forming units kb kilobase pairs L-broth Luria-Bertani medium (see MATERIALS AND METHODS, section a) |
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