An approach to the production of helminth antigens in vitro: The formation of hybrid cells between Fasciola hepatic a and a rat fibroblast cell line |
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Authors: | MJ Howell |
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Institution: | Department of Zoology, Australian National University, Box 4, PO, Canberra, ACT 2600, Australia |
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Abstract: | Using polyethylene glycol, hybrid cells were formed between rat fibroblasts lacking the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT, B.C. 2.4.2.8) and cells of the liver fluke Fasciola hepatica. The hybrid cells survived in a medium containing hypoxanthine, aminopterin and thymidine (HAT) indicating that the enzyme deficiency of the parental rat cells had been corrected. Isoelectric focusing in agarose gels showed that the HGPRT activity in the hybrids was of F. hepatica rather than rat origin. F. hepatica chromosomes could not be identified with certainty in hybrids; and fluke antigens, other than HGPRT, could not be detected in them or in culture medium in which they had grown. |
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Keywords: | rat fibroblasts hypoxanthine-guanine phosphoribosyl transferase HAT medium cell hybridisation karyotypes isoelectric focusing antigens |
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