Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci |
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| Authors: | Karen M Léon-Kloosterziel Marta Alvarez Gil Gerda J Ruijs Steven E Jacobsen Neil E Olszewski Steven H Schwartz Jan AD Zeevaart Maarten Koornneef |
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| Institution: | Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, 6703 HA, Wageningen, The Netherlands;Department of Plant Biology, University of Minnesota, St Paul, MN 55108, USA;MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA |
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| Abstract: | Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3 , that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations. |
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