Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approach |
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| Authors: | Marc G Aucoin Virginie McMurray-Beaulieu Frédéric Poulin Eric B Boivin Jingkui Chen Francisc M Ardelean Mathieu Cloutier Young J Choi Carlos B Miguez and Mario Jolicoeur |
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| Institution: | (1) Canada Research Chair on the Development of Metabolic Engineering Tools, Bio-P2 Research Unit, Department of Chemical Engineering, Ecole Polytechnique de Montr?al, P.O. Box 6079, Centre-Ville Station Montreal, Quebec, Canada;(2) Biotechnology Research Institute, National Research Council of Canada, Royalmount, 6100 Montreal, Quebec, Canada |
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| Abstract: | Background In the interest of generating large amounts of recombinant protein, inducible systems have been studied to maximize both the
growth of the culture and the production of foreign proteins. Even though thermo-inducible systems were developed in the late
1970's, the number of studies that focus on strategies for the implementation at bioreactor scale is limited. In this work,
the bacteriophage lambda PL promoter is once again investigated as an inducible element but for the production of green fluorescent protein (GFP). Culture
temperature, induction point, induction duration and number of inductions were considered as factors to maximize GFP production
in a 20-L bioreactor. |
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