Simple and efficient cell disruption of extremely small quantities of mycelium of phytopathogenic mycotoxin-producing moulds for quantitative extraction of genomic DNA |
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Authors: | V. Saß J. Milles J. Krämer A. Prange |
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Affiliation: | 1. Institut für Ern?hrungs- und Lebensmittelwissenschaften, Lebensmittel-Mikrobiologie und Hygiene, Rheinische Friedrich-Wilhelms-Universit?t Bonn, Meckenheimer Allee 168, 53115, Bonn, Germany
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Abstract: | DNA was efficiently and quantitatively isolated from extremely small quantities of mycelia (0.1–10 mg) of different phytopathogenic moulds by grinding freeze-dried mycelia with glass beads and then using a commercial DNA extraction kit. The efficiency of disruption of the mycelia and the quantitative DNA extraction was proved by microscopy and the quantification of isolated DNA by real time PCR. Presented at the 27th Mykotoxin-Workshop, Dortmund, Germany, June 13–15, 2005 Financial support: German Research Foundation (DFG grant Pr 708/2). J.M. thanks the Cusanuswerk for a doctoral scholarship |
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Keywords: | DNA extraction phytopathogenic moulds quantitative PCR |
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