Spontaneous cell-mediated cytotoxicity (SCMC) and antibody-dependent cellular cytotoxicity (ADCC) in peripheral blood and draining lymph nodes of patients with mammary carcinoma |
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| Authors: | W Heidenreich K Jagla J Schüssler P Börner F Dehnhard J R Kalden W Leibold H H Peter H Deicher |
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| Institution: | (1) Medizinische Hochschule Hannover, Frauenklinik, Podbielskistraße 380, D-3000 Hannover 51;(2) Department of Internal Medicine and Clinical Immunology, Institute of Immunology, Universität Erlangen-Nürnberg, Krankenhausstraße 12, D-8520 Erlangen;(3) Hannover Veterinary School, Institute of Pathology, Bischofsholer Damm 15, D-3000 Hannover;(4) Division of Clinical Immunology and Blood Transfusion, Medizinische Hochschule Hannover, Karl-Wiechert-Allee 9, D-3000 Hannover 61, Federal Republic of Germany |
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| Abstract: | Summary In 14 patients with primary invasive mammary carcinoma (T1–3N0–1M0) lymphocyte preparations obtained from peripheral blood (PBL) and tumor-free or metastatic lymph nodes (LNC) were examined for spontaneous (SCMC) and antibody-dependent cellular cytotoxicity (ADCC) against the allogeneic melanoma cell line IGR3 and a thymoma cell line THY. The cytotoxic activities were compared with those of PBL from healthy women and of LNC from normal mesenteric lymph nodes. In addition, the percentages of E-, EA-, EAC-rosette-forming cells and of surface Ig (SIg)-positive cells were determined for all PBL and LNC suspensions tested.As a rule, LNC exhibited significantly lower SCMC and ADCC than the corresponding PBL preparations. The difference was particularly pronounced in ADCC assays, due to a strikingly low K-cell activity of LNC cells. Consonant with this observation was a reduced percentage of Fc-receptor-bearing cells in LNC suspensions. In SCMC assays the IRG3 targets used in three tests appeared to be less susceptible to LNC effectors than THY targets. No difference in cytotoxicity was noted between PBL from breast cancer patients and from normal women; nor did LNC from tumor-free or metastatic axillary nodes and normal LNC from mesenteric nodes show a significantly different degree of SCMC and ADCC.
Abbreviations used in this paper: ADCC, antibody dependent cellular cytotoxicity; E, neuraminidase treated sheep red blood cells; EAox, ox erythrocytes sensitized with rabbit-anti-ox IgG; EAh, AB erythrocytes sensitized with anti M and N antiserum; EAC, ox erythrocytes sensitized with rabbit-anti-ox IgM and sublytic human complement; LNC, lymph node cells; MEM-FCS, minimal essential medium supplemented with heated fetal calf serum, antibiotics, etc.; NK, de]natural killing; PBL, peripheral blood lymphocytes; PBS, phosphate-buffered saline; SIg, surface immunoglobulin; SCMC, spontaneous cell-mediated cytotoxicity |
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