Cloning and expression analysis of a MAPKKK gene and a novel nodulin gene of Lotus japonicus |
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Authors: | Kinoshita Natsuko Ooki Yasuhiro Deguchi Yuichi Chechetka Svetlana A Kouchi Hiroshi Umehara Yosuke Izui Katsura Hata Shingo |
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Affiliation: | Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. |
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Abstract: | We isolated a cDNA encoding mitogen-activated protein kinase kinase kinase alpha, designated LjM3Kalpha, from Lotus japonicus, a model legume. The gene was expressed constitutively in roots, root nodules, and shoots. We also identified a novel nodulin gene, LjNUF, that shows specific expression in nodules. LjNUF resembles the C-terminal half of a hypothetical protein (pir//D85436), the N-terminal half of which is similar to a portion of mitogen-activated protein kinase kinase kinase gamma. Although LjNUF was predicted to be a secreted protein, its function remains to be clarified. |
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