miR-148a-3p promotes rabbit preadipocyte differentiation by targeting PTEN |
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Authors: | Hongbing He Mingcheng Cai Jiaoyue Zhu Wudian Xiao Buwei Liu Yu Shi Xue Yang Xiaohu Liang Tianhao Zheng Shenqiang Hu Xianbo Jia Shiyi Chen Jie Wang Yinghe Qin Songjia Lai |
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Affiliation: | 1.Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Sichuan,China;2.College of Animal Science and Technology,China Agricultural University,Haidian District,People’s Republic of China;3.Chendu Academy of Agriculture and Forestry Sciences,Sichuan,China |
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Abstract: | Although emerging data support crucial roles for microRNAs (miRNAs) during adipogenesis, the detailed mechanisms remain largely unknown. In this study, it was shown that in rabbits, levels of miR-148a-3p not only increased in white adipose tissue during early stages of growth but also during in vitro cultured preadipocyte differentiation. Furthermore, overexpression of miR-148a-3p significantly upregulated the mRNA levels of PPARγ, C/EBPα, and FABP4, as well as the protein levels of PPARγ, as indicated by qPCR and western blotting analyses. Overexpression of miR-148a-3p also promoted intracellular triglyceride accumulation. In contrast, downregulation of miR-148a-3p inhibited the differentiation of rabbit preadipocytes. Next, based on target gene prediction and a luciferase reporter assay, we further demonstrated that miR-148a-3p directly targeted one of the 3′ untranslated regions of PTEN. Finally, it was observed inhibition of PTEN by siRNA promoted rabbit preadipocyte differentiation. Taken together, our results suggested that miR-148a-3p could be involved in regulating rabbit preadipocyte differentiation through inhibiting expression of PTEN, which further highlighted the importance of miRNAs during adipogenesis. |
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