A rapid enzymatic procedure for production of 1-beta-D-(3H)arabinofuranosyl cytosine 5'-triphosphate. |
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| Authors: | C G Mintz C Agent N B Furlong |
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| Affiliation: | 1. The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030 USA;2. The University of Texas Health Science Center Graduate School of Biomedical Sciences, Houston, Texas 77030 USA |
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| Abstract: | A rapid method of sequentially phosphorylating picomole quantities of [3H]-araC to [3H]araCTP is described (ara = 1-β-d-arabinofuranosyl). The procedure utilizes a system of phosphorylating enzymes isolated from rat spleen and requires a single incubation step. The [3H]araCTP product is isolated by ion-exchange chromatography and analyzed by PEI-cellulose thin-layer chromatography. At low concentrations of [3H]araC as much as 80% can be phosphorylated to the triphosphate, and the produet may be obtained in radiochemical purity greater than 97%. |
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| Keywords: | Requests for reprints should be sent to N. Burr Furlong Department of Biochemistry M. D. Anderson Hospital and Tumor Institute 6723 Bertner Avenue Houston Tex. 77030. |
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