Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P.O. Box 5000, University of Oulu, FIN-90014 Oulu, Finland.