A nondestructive,rapid, reliable and inexpensive method to sample,store and extract high‐quality DNA from fish body mucus and buccal cells |
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Authors: | LUCENTINI LIVIA PALOMBA ANTONELLA LANCIONI HOVIRAG NATALI MAURO FAUSTO PANARA |
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Abstract: | A rapid, nondestructive, reproducible and cheap DNA extraction method from body mucus and buccal cells of northern pike and brown trout is described. Buccal cells and body mucus were sampled on FTA Cards; the captured DNA was used directly for microsatellite and polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analyses. A complete concordance with control DNA was found. The genotyping error rate for microsatellite ranged from 1.9% to 3.3% for the northern pike and brown trout, respectively. This methodology, using for the first time these materials as a fish DNA source, combines speed of sampling and processing, with a twofold to a threefold time and costs saving. |
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Keywords: | fish body mucus fish buccal cells Nondestructive |
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