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Characterization of di‐, tri‐ and tetranucleotide microsatellite markers with perfect repeats for Trypanosoma brucei and related species
Authors:OLIVER BALMER  CHRISTOPHER PALMA  ANNETTE MACLEOD  ADALGISA CACCONE
Abstract:
Trypanosoma brucei, a unicellular parasite causing human sleeping sickness and animal nagana, has a great impact on the socioeconomic environment of sub‐Saharan Africa. The dynamics of the parasite are still poorly understood. We have characterized 14 polymorphic di‐, tri‐ and tetranucleotide microsatellite loci with perfect repeats (only one motif) exhibiting between five and 16 alleles in T. brucei isolates from all over Africa and from all described subspecies. The microsatellites will be useful in addressing population genetic questions in T. brucei to better understand the population structure and spread of this important parasite.
Keywords:coinfection  cross‐amplification  kinetoplastid  microsatellites  population genetics  Trypanosoma
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