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Development and polymorphisms of microsatellite markers for hinoki (Chamaecyparis obtusa)
Authors:ASAKO MATSUMOTO  NAOKI TANI  XIN‐GUO LI  YURI NAKAO  NOBUHIRO TOMARU  YOSHIHIKO TSUMURA
Abstract:Fifteen microsatellite markers were developed from a microsatellite‐enriched library and characterized using 32 Chamaecyparis obtusa individuals. The number of alleles ranged from two to 27 per locus, with observed heterozygosity ranging from 0.281 to 0.906. The polymorphism information content (PIC) was also calculated for each marker and the average was 0.796 ± 0.024. These microsatellite markers will be useful for investigating population genetics, reproductive ecology, tree improvement and constructing linkage maps of the species.
Keywords:Chamaecyparis obtusa  conifer  Cupressaceae  microsatellite
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