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Quality indexes to assess the reliability of genotypes in studies using noninvasive sampling and multiple‐tube approach
Authors:C. MIQUEL,E. BELLEMAIN,C. POILLOT,J. BESSI   RE,A. DURAND,P. TABERLET
Affiliation:C. MIQUEL,E. BELLEMAIN,C. POILLOT,J. BESSIÈRE,A. DURAND,P. TABERLET
Abstract:In noninvasive studies, the intersample variance in DNA quality and quantity is large, and produces multilocus genotypes of highly variable quality. Here we propose a standardized method for testing the reliability of the genotyping procedure when using the multiple‐tube approach. The quality indexes generated will allow reliable comparisons among samples, loci, studies, and field and/or laboratory protocols. These indexes represent a powerful tool for the quality management of noninvasive studies.
Keywords:genotyping errors  microsatellites  noninvasive samples  quality index
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