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Polymorphic microsatellite markers isolated from partially enriched genomic library of Chitala chitala
Authors:PEYUSH PUNIA  HARI SHANKAR GUPTA  RAJEEV K SINGH  VINDHYA MOHINDRA  KULDEEP K LAL    V S CHAUHAN  W S LAKRA
Abstract:A total eight polymorphic microsatellite loci were obtained from genomic library of Indian feather back, Chitala chitala (order Osteoglossiformes, family Notopteridae) and the 46 samples were analysed to determine genetic variation. The mean number of alleles per locus ranged from 4.50 to 5.25, and expected heterozygosity ranged from 0.124 to 0.852. Deviation from Hardy–Weinberg equilibrium expectations (P < 0.002) was observed at loci Cch2, Cch9 (Bhaghirathi) and Cch9 (Brahmaputra). The identified microsatellite loci were found promising for population genetics studies of C. chitala and related species Notopterus notopterus (family Notopteridae).
Keywords:Chitala chitala  microsatellite  Notopterus notopterus  null allele  polymorphic  population genetics
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