A simple method for <Emphasis Type="Italic">in vivo</Emphasis> labelling of lipid fractions in developing seeds of <Emphasis Type="Italic">Brassica campestris</Emphasis> L |
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Authors: | Niti?Sharma Archana Sarla?P?Malhotra Email author" target="_blank">Randhir?SinghEmail author |
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Institution: | (1) Plant Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, CCS Haryana Agricultural University, 125 004 Hisar, India |
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Abstract: | An in vivo method of labelling lipid fractions in developing seeds of Brassica campestris using 1–14C] acetate has been developed. The “wick” method for introducing label into the intact plant is quite effective, safe and
easy to use. The results obtained were reproducible and comparable to those reported earlier for seeds procured from greenhouse
grown plants. The labelling pattern showed that rapid oil deposition began around 20 days after anthesis (DAA) and continued
until about 45 DAA. The proportion of label in polar lipids declined and that in non-polar lipids increased during the phase
of active oil synthesis. Among phospholipids, the label was incorporated mainly in phosphatidyl choline (PC), which was found
to be the major fraction of phospholipids. During development, the two galactolipids i.e. monogalactosyl diglyceride (MGDG)
and digalactosyl diglyceride (DGDG) followed patterns exactly opposite to each other. The content of the label in MGDG decreased,
while that in DGDG increased, indicating the conversion of MGDG to DGDG during maturation. |
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Keywords: | Brassica campestris developing seeds lipid fractions in vivo labelling |
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