Immunostimulatory principles from Chlorella pyrenoidosa—Part 1: Isolation and biological assessment in vitro |
| |
Authors: | JA Kralovec KL Metera JR Kumar LV Watson GS Girouard Y Guan RI Carr CJ Barrow HS Ewart |
| |
Institution: | Ocean Nutrition Canada Ltd., 101 Research Drive, Dartmouth, NS, B2Y 4T6. jkralovec@ocean-nutrition.com |
| |
Abstract: | Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose. |
| |
Keywords: | Chlorella extract Bioassay-guided fractionation Immunostimulatory B cells Macrophages |
本文献已被 ScienceDirect 等数据库收录! |