Isolation and characterization of Sphingomonas sp. GTIN11 capable of carbazole metabolism in petroleum |
| |
Authors: | Kilbane John J Daram Amrutha Abbasian Javaneh Kayser Kevin J |
| |
Institution: | Gas Technology Institute, Des Plaines, IL 60018, USA. john.kilbane@gastechnology.org |
| |
Abstract: | A bacterial culture was isolated from a manufactured gas plant (MGP) soil based on its ability to metabolize the nitrogen-containing heterocycle carbazole. The culture was identified as a Sphingomonas sp. and was given the designation GTIN11. A cloned 4.2kb DNA fragment was confirmed to contain genes responsible for carbazole degradation. DNA sequence analysis revealed that the fragment contained five open reading frames (ORFs) with the deduced amino acid sequence showing homology to; carbazole terminal dioxygenase (ORF1), 2,3-dihydroxybiphenyl dioxygenase subunits (ORF2 and ORF3), meta-cleavage compound hydrolases (ORF4), and ferrodoxin component of bacterial multicomponent dioxygenases (ORF5). The percent similarity was 61% of these proteins or less to known proteins. The specific activity of Sphingomonas sp. GTIN11 for the degradation of carbazole at 37 degrees C was determined to be 8.0 micromol carbazole degraded/min/g dry cell. This strain is unique in expressing the carbazole degradation trait constitutively. Resting cells of Sphingomonas sp. GTIN11 removed 95% of carbazole and 50% of C1-carbazoles from petroleum in a 16-h treatment time. |
| |
Keywords: | Carbazole Organonitrogen Biodegradation Petroleum Shale oil |
本文献已被 ScienceDirect PubMed 等数据库收录! |