| Inhibition of the activating signals in NK92 cells by recombinant GST-sHLA-G1α chain |
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| 引用本文： | Yao AY,Tang HY,Wang Y,Feng MF,Zhou RL. Inhibition of the activating signals in NK92 cells by recombinant GST-sHLA-G1α chain[J]. Cell research, 2004, 14(2): 155-160 |
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| 作者姓名： | Yao AY Tang HY Wang Y Feng MF Zhou RL |
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| 作者单位： | [1]StateKeyLaboratoryofBiomembraneandMembraneBiotechnology,InstituteofZoology,ChineseAcademyofScience,Beijing100080,China [2]DepartmentofCellBiologyandMedicalGenetics,SchoolofBasicMedicalSciences,HealthScienceCenter,PekingUniversity,Beijing100083,China. |
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| 摘 要： | The soluble HLA-G1 (sHLA-G1) isoform was found to be secreted by trophoblast cells at the materno-fetal interface,which suggests that it may act as an immunomodulator during pregnancy. In this paper, we reported that GST-sHLA-G1α chain could bind to its receptor ILT-2 on NK92 cells and then the latter recruited Src homology 2 domaincontaining tyrosine phosphatase-1 (SHP-1), which consequently dephosphorylated some important protein tyrosine kinases and blocked the activation of downstream molecules such as MEK and ERK so that the cytotoxicity of natural killer (NK) cells was inhibited. These results indicated that GST-sHLA-G1α chain might be exploited in new immunotherapy strategies aiming at inducing immunotolerance during allograft, xenograft and autoimmune situations. In addition,we found that modification of O-linked β-N-acetylglucosamine (O-GlcNAc) was involved in NK cells‘ activating and inhibitory signals. This may provide a novel molecular target for inducing immunotolerance but needs further study.
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| 关 键 词： | GST-sHLA-G1α 活化信号 抑制 NK92 细胞 重组体 信号转导 对碘氧基苯甲醚 免疫调节剂 |
Inhibition of the activating signals in NK92 cells by recombinant GST-sHLA-G1a chain |
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| Yao Ai Yu,Tang Hai Yang,Wang Yun,Feng Mei Fu,Zhou Rou Lin. Inhibition of the activating signals in NK92 cells by recombinant GST-sHLA-G1a chain[J]. Cell research, 2004, 14(2): 155-160 |
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| Authors: | Yao Ai Yu Tang Hai Yang Wang Yun Feng Mei Fu Zhou Rou Lin |
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| Affiliation: | State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Science, Beijing 100080, China. |
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| Abstract: | The soluble HLA-G1 (sHLA-G1) isoform was found to be secreted by trophoblast cells at the materno-fetal interface, which suggests that it may act as an immunomodulator during pregnancy. In this paper, we reported that GST-sHLA-G1a chain could bind to its receptor ILT-2 on NK92 cells and then the latter recruited Src homology 2 domain-containing tyrosine phosphatase-1 (SHP-1), which consequently dephosphorylated some important protein tyrosine kinases and blocked the activation of downstream molecules such as MEK and ERK so that the cytotoxicity of natural killer (NK) cells was inhibited. These results indicated that GST-sHLA-G1a chain might be exploited in new immunotherapy strategies aiming at inducing immunotolerance during allograft, xenograft and autoimmune situations. In addition, we found that modification of O-linked b-N-acetylglucosamine (O-GlcNAc) was involved in NK cells' activating and inhibitory signals. This may provide a novel molecular target for inducing immunotolerance but needs further study. |
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