H2 production from chemostat fermentation of glucose byClostridium butyricum andClostridium pasteurianum in ammonium- and phosphate limitation |
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Authors: | M Heyndrickx P De Vos J De Ley |
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Institution: | (1) Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium |
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Abstract: | Summary In ammonium-limitation (4.55 mM NH4
+) at a dilution rate (D)=0.081 h–1,Clostridium butyricum produced 2 mol H2 per mol glucose consumed at pH 5.0, but at a low fermentation rate. At higher pH, important amounts of extracellular protein were produced. Phosphatelimitation (0.5 mM PO4
–3) at D=0.061 h–1 and pH 7.0 were the best conditions tested for hydrogen gas production (2.22 mol H2 per mol glucose consumed) at a high fermentation rate. Steady-state growth at lower pH and with 0.1 mM PO4
–3 resulted in proportional higher glucose incorporation into biomass and lower H2 production.
C. pasteurianum in NH4
+ limitation showed higher fermentation rates thanC. butyricum and a stabilized H2 production around 2.08 (±0.06) mol per mol glucose consumed at various defined pH conditions, although the acetate/butyrate ratio increased to 1 at pH 7.0. The latter was also observed in phosphate-limitation, but here H2 production was maximal (1.90 mol. per mol glucose consumed) at the lowest pH (5.5) tested. |
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