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Use of antisera to localize callose,xylan and arabinogalactan in the cell-plate,primary and secondary walls of plant cells
Authors:D. H. Northcote  Robin Davey  Jim Lay
Affiliation:(1) Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW Cambridge, UK
Abstract:Antibodies to cellobiose, L-arabinopyranose, L-arabinofuranose, D-galactose, oligosaccharides containing beta1rarr4 xylose, oligosaccharides containing beta1rarr4 glucose, and oligosaccharides containing beta1rarr3 glucose have been raised in rabbits. The antisera have been characterized to show the specificity of binding to particular polysaccharides. They have been used for immunocytology using the electron microscope to locate the polymers in dividing and differentiating cells of bean (Phaseolus vulgaris L.) root, bean callus tissue and cells of Zinnia elegans L. in vitro. Arabinogalactans have been shown to be present in the cell-plate and primary walls but not in secondary thickening. Xylan as distinct from xyloglucan was found in the primary walls but not in the cell-plate. It was present in large amounts in the secondary thickening. Callose was found in the cell plate and also in the young growing wall. In the wall it was specifically located at the plasmodesmata. The use of the antibody against L-arabinofuranose enabled a specific organelle to be detected which was membranous and which occurred within the cytoplasm and also within the vacuole of the cells. Membranes carrying polymers containing L-arabinofuranose were also found in layers just under the plasmamembrane.Abbreviations L-Araf L-arabinofuranose - L-Arap L-arabinopyranose - BSA bovine serum albumin - Gal galactose - D-Galp D-galactopyranose - Glc glucose - Xyl xylose
Keywords:Cell culture (cell-wall constituents)  Cell wall  Immunocytology  Peetin secondary thickening  Phaseolus (cell wall)  Xylem elements  Zinnia
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