Efficient selection for thermostable protease in Thermus thermophilus |
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Authors: | Takagi H Suzumura A Hasuura Y Hoshino T Nakamori S |
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Affiliation: | Department of Bioscience, Fukui Prefectural University, Japan. hiro@fpu.ac.jp |
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Abstract: | An efficient procedure was established to select for thermostable proteases in an extreme thermophile, Thermus thermophilus. A non-protease-secreting mutant derived from T. thermophilus TH125 was used as host and the expression plasmid for aqualysin I from T. aquaticus YT-1 was constructed as a source of thermostable protease. T. thermophilus cells harboring the recombinant plasmid produced active aqualysin I into the medium and were able to grow on a minimal medium containing milk casein as the sole source of carbon and nitrogen. |
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