Peroxidatic activity in heart effluent: a biochemical parameter for the assessment of experimental ischemia-reperfusion injury |
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| Authors: | Leabu M. V. Bucur Elisa A. |
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| Affiliation: | Department of Cell Biology and Histology, "Carol Davila" University of Medicine and Pharmacy, 8 Eroilor Sanitari Blvd, 76241 Bucharest, Romania |
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| Abstract: | Peroxidatic activity in heart effluent was defined as a new biochemical parameter for the experimental study of myocardial ischemia. The peroxidatic reaction was determined by dot blot analysis with 3,3'-diaminobenzidine as hydrogen donor. After ischemia, the level of peroxidatic activity in heart effluent was 2-3 times higher than before. The effects in experimental modulation of ischemia, such as nicorandil or aprikalim protection, and the reversibility of protection by glibenclamide, could accurately be noted using the level of peroxidatic activity in heart effluent as a biochemical parameter. The results were in good agreement with those obtained for other enzymes used as biochemical parameters in experimental heart ischemia-reperfusion studies. |
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| Keywords: | heart Langendorff preparation ischemia-reperfusion nicorandil aprikalim glibenclamide peroxidatic activity |
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