Cytosine Deaminase as a Negative Selection Marker for Gene Disruption and Replacement in the Genus Streptomyces and Other Actinobacteria |
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| Authors: | Marie-Pierre Dubeau Mariana Gabriela Ghinet Pierre-tienne Jacques Nancy Clermont Carole Beaulieu and Ryszard Brzezinski |
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| Institution: | Marie-Pierre Dubeau, Mariana Gabriela Ghinet, Pierre-Étienne Jacques, Nancy Clermont, Carole Beaulieu, and Ryszard Brzezinski |
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| Abstract: | We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein from Escherichia coli optimized for expression in Streptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors. |
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